Review



expression plasmids encoding memerald tagged gfap  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 92
      Buy from Supplier

    Structured Review

    Addgene inc expression plasmids encoding memerald tagged gfap
    Fluorescent scFv immuno-probes generated in this work
    Expression Plasmids Encoding Memerald Tagged Gfap, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expression plasmids encoding memerald tagged gfap/product/Addgene inc
    Average 92 stars, based on 2 article reviews
    expression plasmids encoding memerald tagged gfap - by Bioz Stars, 2026-03
    92/100 stars

    Images

    1) Product Images from "Multiplexed volumetric CLEM enabled by scFvs provides insights into the cytology of cerebellar cortex"

    Article Title: Multiplexed volumetric CLEM enabled by scFvs provides insights into the cytology of cerebellar cortex

    Journal: Nature Communications

    doi: 10.1038/s41467-024-50411-z

    Fluorescent scFv immuno-probes generated in this work
    Figure Legend Snippet: Fluorescent scFv immuno-probes generated in this work

    Techniques Used: Generated, Expressing, Plasmid Preparation

    a Representative confocal images ( n = 3 experiments in each category) of different sections from the cerebellum labeled with: a calbindin-specific scFv probe conjugated with Alexa Fluor 488, a VGluT1-specific scFv probe conjugated with Alexa Fluor 532, a GFAP-specific scFv probe conjugated with 5-TAMRA, a K v 1.2-specific scFv probe conjugated with Alexa Fluor 594, and a parvalbumin-specific scFv probe conjugated with Alexa Fluor 647. The double dotted lines delineate the Purkinje cell layer. (see Supplementary Figs. and for larger fields of view). CB calbindin, VGluT1 vesicular glutamate transporter 1, GFAP glial fibrillary acidic protein, K v 1.2 potassium voltage-gated channel subfamily A member 2, PV parvalbumin, TAM 5-TAMRA. b Workflow of multicolor imaging enabled by scFv probes and linear unmixing (see the text). c Representative maximum intensity projection of the multicolor fluorescence image stack acquired by linear unmixing of confocal images ( n = 3 experiments). The signal of each fluorescent dye was pseudo-colored for better visualization. d Enlarged boxed inset from ( c ). The arrow indicates a Bergmann fiber (GFAP-positive) adjacent to the main dendrite of a Purkinje cell. Arrowhead indicates sites where axons form a pinceau structure labeled by the K v 1.2-specific scFv probe.
    Figure Legend Snippet: a Representative confocal images ( n = 3 experiments in each category) of different sections from the cerebellum labeled with: a calbindin-specific scFv probe conjugated with Alexa Fluor 488, a VGluT1-specific scFv probe conjugated with Alexa Fluor 532, a GFAP-specific scFv probe conjugated with 5-TAMRA, a K v 1.2-specific scFv probe conjugated with Alexa Fluor 594, and a parvalbumin-specific scFv probe conjugated with Alexa Fluor 647. The double dotted lines delineate the Purkinje cell layer. (see Supplementary Figs. and for larger fields of view). CB calbindin, VGluT1 vesicular glutamate transporter 1, GFAP glial fibrillary acidic protein, K v 1.2 potassium voltage-gated channel subfamily A member 2, PV parvalbumin, TAM 5-TAMRA. b Workflow of multicolor imaging enabled by scFv probes and linear unmixing (see the text). c Representative maximum intensity projection of the multicolor fluorescence image stack acquired by linear unmixing of confocal images ( n = 3 experiments). The signal of each fluorescent dye was pseudo-colored for better visualization. d Enlarged boxed inset from ( c ). The arrow indicates a Bergmann fiber (GFAP-positive) adjacent to the main dendrite of a Purkinje cell. Arrowhead indicates sites where axons form a pinceau structure labeled by the K v 1.2-specific scFv probe.

    Techniques Used: Labeling, Imaging, Fluorescence

    a The high-resolution EM volume acquired from the cerebellar lobule, Crus 1 with multicolor immunofluorescence from scFv probes separated by linear unmixing ( n = 1 experiment). The multicolor fluorescence data was co-registered with the high-resolution EM data. The Neuroglancer link to access the dataset is provided in the source data file. Numbers 1–4 indicate approximate regions where the ultrastructure was examined at high resolution ( n = 12 experiments). Owing to the absence of detergent in immunofluorescence labeling, fine ultrastructure was preserved throughout the EM volume, such as in the molecular layer (1), in the Purkinje cell layer (2), in the glomeruli in the granule cell layer (3), and in the granule cell bodies (4). b Demonstration of the overlay between fluorescence signals and EM ultrastructure. Left panel shows the multicolor six-channel fluorescent image of slice 250 ( n = 848 slices) of the spatially transformed fluorescence image volume. The middle panel shows three fluorescence channels corresponding to the labeling of CB, GFAP, and Hoechst overlaid onto the EM micrograph of slice 250. Right panel shows four fluorescent channels corresponding to the labeling of VGluT1, K v 1.2, PV, and Hoechst overlaid onto the EM micrograph of slice 250. Other examples of fluorescence overlay are shown in Supplementary Fig. .
    Figure Legend Snippet: a The high-resolution EM volume acquired from the cerebellar lobule, Crus 1 with multicolor immunofluorescence from scFv probes separated by linear unmixing ( n = 1 experiment). The multicolor fluorescence data was co-registered with the high-resolution EM data. The Neuroglancer link to access the dataset is provided in the source data file. Numbers 1–4 indicate approximate regions where the ultrastructure was examined at high resolution ( n = 12 experiments). Owing to the absence of detergent in immunofluorescence labeling, fine ultrastructure was preserved throughout the EM volume, such as in the molecular layer (1), in the Purkinje cell layer (2), in the glomeruli in the granule cell layer (3), and in the granule cell bodies (4). b Demonstration of the overlay between fluorescence signals and EM ultrastructure. Left panel shows the multicolor six-channel fluorescent image of slice 250 ( n = 848 slices) of the spatially transformed fluorescence image volume. The middle panel shows three fluorescence channels corresponding to the labeling of CB, GFAP, and Hoechst overlaid onto the EM micrograph of slice 250. Right panel shows four fluorescent channels corresponding to the labeling of VGluT1, K v 1.2, PV, and Hoechst overlaid onto the EM micrograph of slice 250. Other examples of fluorescence overlay are shown in Supplementary Fig. .

    Techniques Used: Immunofluorescence, Fluorescence, Labeling, Transformation Assay

    a 2D CLEM image showing the fluorescence signal (green) of the calbindin-specific scFv probe overlapping with the cell body of a Purkinje cell. b EM image showing 2D segmentation (green) of the calbindin-positive Purkinje cell ( n = 1). c 3D reconstruction of the Purkinje cell labeled in a ( n = 1), with the cell body in dark green and a dendritic branch in light green; three parallel fibers (red) make synapses on three spine heads of the dendritic branch (arrow indicates a parallel fiber (PF); arrowhead indicates a synapse). d EM image showing 2D segmentation of the synapse (arrowhead) between a parallel fiber (red) and a spine head of the dendritic branch (green) ( n = 1). e 2D CLEM image showing fluorescence signals (red) of the GFAP-specific scFv probe overlapping with the cell body of a velate astrocyte in the granule cell layer ( n = 1). f EM image showing 2D segmentation (red) of the velate astrocyte in ( e ) ( n = 1). g 3D reconstruction of the velate astrocyte (red) labeled in ( e ) and two nearby granule cells (GC1 and GC2, light and dark blue) ( n = 2); the astrocyte extends a veil-like glial process (arrowhead) between the two granule cells. h EM image showing 2D segmentation of the glial process (arrowhead) between GC1 and GC2 ( n = 1). i 2D CLEM image showing fluorescence signals (red) of the GFAP-specific scFv probe overlapping with a Bergmann fiber ( n = 1). j EM image showing 2D segmentation (red) of the Bergmann fiber in ( i ) ( n = 1). k 3D reconstruction of two Bergmann glial cells (BG1 and BG2) ( n = 2) traced from their Bergmann fibers labeled by the GFAP-specific scFv probe. l EM image showing 2D segmentation of the cell body of BG2 ( n = 1) and a nearby basket cell ( n = 1), noting the lack of infoldings in BG2’s nuclear membrane compared to the basket cell. n indicates an example.
    Figure Legend Snippet: a 2D CLEM image showing the fluorescence signal (green) of the calbindin-specific scFv probe overlapping with the cell body of a Purkinje cell. b EM image showing 2D segmentation (green) of the calbindin-positive Purkinje cell ( n = 1). c 3D reconstruction of the Purkinje cell labeled in a ( n = 1), with the cell body in dark green and a dendritic branch in light green; three parallel fibers (red) make synapses on three spine heads of the dendritic branch (arrow indicates a parallel fiber (PF); arrowhead indicates a synapse). d EM image showing 2D segmentation of the synapse (arrowhead) between a parallel fiber (red) and a spine head of the dendritic branch (green) ( n = 1). e 2D CLEM image showing fluorescence signals (red) of the GFAP-specific scFv probe overlapping with the cell body of a velate astrocyte in the granule cell layer ( n = 1). f EM image showing 2D segmentation (red) of the velate astrocyte in ( e ) ( n = 1). g 3D reconstruction of the velate astrocyte (red) labeled in ( e ) and two nearby granule cells (GC1 and GC2, light and dark blue) ( n = 2); the astrocyte extends a veil-like glial process (arrowhead) between the two granule cells. h EM image showing 2D segmentation of the glial process (arrowhead) between GC1 and GC2 ( n = 1). i 2D CLEM image showing fluorescence signals (red) of the GFAP-specific scFv probe overlapping with a Bergmann fiber ( n = 1). j EM image showing 2D segmentation (red) of the Bergmann fiber in ( i ) ( n = 1). k 3D reconstruction of two Bergmann glial cells (BG1 and BG2) ( n = 2) traced from their Bergmann fibers labeled by the GFAP-specific scFv probe. l EM image showing 2D segmentation of the cell body of BG2 ( n = 1) and a nearby basket cell ( n = 1), noting the lack of infoldings in BG2’s nuclear membrane compared to the basket cell. n indicates an example.

    Techniques Used: Fluorescence, Labeling, Membrane



    Similar Products

    expression plasmids encoding memerald tagged gfap  (Addgene inc)
    92
    Addgene inc expression plasmids encoding memerald tagged gfap
    Fluorescent scFv immuno-probes generated in this work
    Expression Plasmids Encoding Memerald Tagged Gfap, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expression plasmids encoding memerald tagged gfap/product/Addgene inc
    Average 92 stars, based on 1 article reviews
    expression plasmids encoding memerald tagged gfap - by Bioz Stars, 2026-03
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    Fluorescent scFv immuno-probes generated in this work

    Journal: Nature Communications

    Article Title: Multiplexed volumetric CLEM enabled by scFvs provides insights into the cytology of cerebellar cortex

    doi: 10.1038/s41467-024-50411-z

    Figure Lengend Snippet: Fluorescent scFv immuno-probes generated in this work

    Article Snippet: After adherence, cells were transfected with mammalian expression plasmids encoding mEmerald-tagged GFAP (Addgene #54107 or Flag-tagged human calbindin (Origene # RC201358) using Lipofectamine 2000 (Thermo Fisher # 11668500) or Lipofectamine 3000 (Thermo Fisher # L3000001) transfection reagent following the manufacturer’s protocol.

    Techniques: Generated, Expressing, Plasmid Preparation

    a Representative confocal images ( n = 3 experiments in each category) of different sections from the cerebellum labeled with: a calbindin-specific scFv probe conjugated with Alexa Fluor 488, a VGluT1-specific scFv probe conjugated with Alexa Fluor 532, a GFAP-specific scFv probe conjugated with 5-TAMRA, a K v 1.2-specific scFv probe conjugated with Alexa Fluor 594, and a parvalbumin-specific scFv probe conjugated with Alexa Fluor 647. The double dotted lines delineate the Purkinje cell layer. (see Supplementary Figs. and for larger fields of view). CB calbindin, VGluT1 vesicular glutamate transporter 1, GFAP glial fibrillary acidic protein, K v 1.2 potassium voltage-gated channel subfamily A member 2, PV parvalbumin, TAM 5-TAMRA. b Workflow of multicolor imaging enabled by scFv probes and linear unmixing (see the text). c Representative maximum intensity projection of the multicolor fluorescence image stack acquired by linear unmixing of confocal images ( n = 3 experiments). The signal of each fluorescent dye was pseudo-colored for better visualization. d Enlarged boxed inset from ( c ). The arrow indicates a Bergmann fiber (GFAP-positive) adjacent to the main dendrite of a Purkinje cell. Arrowhead indicates sites where axons form a pinceau structure labeled by the K v 1.2-specific scFv probe.

    Journal: Nature Communications

    Article Title: Multiplexed volumetric CLEM enabled by scFvs provides insights into the cytology of cerebellar cortex

    doi: 10.1038/s41467-024-50411-z

    Figure Lengend Snippet: a Representative confocal images ( n = 3 experiments in each category) of different sections from the cerebellum labeled with: a calbindin-specific scFv probe conjugated with Alexa Fluor 488, a VGluT1-specific scFv probe conjugated with Alexa Fluor 532, a GFAP-specific scFv probe conjugated with 5-TAMRA, a K v 1.2-specific scFv probe conjugated with Alexa Fluor 594, and a parvalbumin-specific scFv probe conjugated with Alexa Fluor 647. The double dotted lines delineate the Purkinje cell layer. (see Supplementary Figs. and for larger fields of view). CB calbindin, VGluT1 vesicular glutamate transporter 1, GFAP glial fibrillary acidic protein, K v 1.2 potassium voltage-gated channel subfamily A member 2, PV parvalbumin, TAM 5-TAMRA. b Workflow of multicolor imaging enabled by scFv probes and linear unmixing (see the text). c Representative maximum intensity projection of the multicolor fluorescence image stack acquired by linear unmixing of confocal images ( n = 3 experiments). The signal of each fluorescent dye was pseudo-colored for better visualization. d Enlarged boxed inset from ( c ). The arrow indicates a Bergmann fiber (GFAP-positive) adjacent to the main dendrite of a Purkinje cell. Arrowhead indicates sites where axons form a pinceau structure labeled by the K v 1.2-specific scFv probe.

    Article Snippet: After adherence, cells were transfected with mammalian expression plasmids encoding mEmerald-tagged GFAP (Addgene #54107 or Flag-tagged human calbindin (Origene # RC201358) using Lipofectamine 2000 (Thermo Fisher # 11668500) or Lipofectamine 3000 (Thermo Fisher # L3000001) transfection reagent following the manufacturer’s protocol.

    Techniques: Labeling, Imaging, Fluorescence

    a The high-resolution EM volume acquired from the cerebellar lobule, Crus 1 with multicolor immunofluorescence from scFv probes separated by linear unmixing ( n = 1 experiment). The multicolor fluorescence data was co-registered with the high-resolution EM data. The Neuroglancer link to access the dataset is provided in the source data file. Numbers 1–4 indicate approximate regions where the ultrastructure was examined at high resolution ( n = 12 experiments). Owing to the absence of detergent in immunofluorescence labeling, fine ultrastructure was preserved throughout the EM volume, such as in the molecular layer (1), in the Purkinje cell layer (2), in the glomeruli in the granule cell layer (3), and in the granule cell bodies (4). b Demonstration of the overlay between fluorescence signals and EM ultrastructure. Left panel shows the multicolor six-channel fluorescent image of slice 250 ( n = 848 slices) of the spatially transformed fluorescence image volume. The middle panel shows three fluorescence channels corresponding to the labeling of CB, GFAP, and Hoechst overlaid onto the EM micrograph of slice 250. Right panel shows four fluorescent channels corresponding to the labeling of VGluT1, K v 1.2, PV, and Hoechst overlaid onto the EM micrograph of slice 250. Other examples of fluorescence overlay are shown in Supplementary Fig. .

    Journal: Nature Communications

    Article Title: Multiplexed volumetric CLEM enabled by scFvs provides insights into the cytology of cerebellar cortex

    doi: 10.1038/s41467-024-50411-z

    Figure Lengend Snippet: a The high-resolution EM volume acquired from the cerebellar lobule, Crus 1 with multicolor immunofluorescence from scFv probes separated by linear unmixing ( n = 1 experiment). The multicolor fluorescence data was co-registered with the high-resolution EM data. The Neuroglancer link to access the dataset is provided in the source data file. Numbers 1–4 indicate approximate regions where the ultrastructure was examined at high resolution ( n = 12 experiments). Owing to the absence of detergent in immunofluorescence labeling, fine ultrastructure was preserved throughout the EM volume, such as in the molecular layer (1), in the Purkinje cell layer (2), in the glomeruli in the granule cell layer (3), and in the granule cell bodies (4). b Demonstration of the overlay between fluorescence signals and EM ultrastructure. Left panel shows the multicolor six-channel fluorescent image of slice 250 ( n = 848 slices) of the spatially transformed fluorescence image volume. The middle panel shows three fluorescence channels corresponding to the labeling of CB, GFAP, and Hoechst overlaid onto the EM micrograph of slice 250. Right panel shows four fluorescent channels corresponding to the labeling of VGluT1, K v 1.2, PV, and Hoechst overlaid onto the EM micrograph of slice 250. Other examples of fluorescence overlay are shown in Supplementary Fig. .

    Article Snippet: After adherence, cells were transfected with mammalian expression plasmids encoding mEmerald-tagged GFAP (Addgene #54107 or Flag-tagged human calbindin (Origene # RC201358) using Lipofectamine 2000 (Thermo Fisher # 11668500) or Lipofectamine 3000 (Thermo Fisher # L3000001) transfection reagent following the manufacturer’s protocol.

    Techniques: Immunofluorescence, Fluorescence, Labeling, Transformation Assay

    a 2D CLEM image showing the fluorescence signal (green) of the calbindin-specific scFv probe overlapping with the cell body of a Purkinje cell. b EM image showing 2D segmentation (green) of the calbindin-positive Purkinje cell ( n = 1). c 3D reconstruction of the Purkinje cell labeled in a ( n = 1), with the cell body in dark green and a dendritic branch in light green; three parallel fibers (red) make synapses on three spine heads of the dendritic branch (arrow indicates a parallel fiber (PF); arrowhead indicates a synapse). d EM image showing 2D segmentation of the synapse (arrowhead) between a parallel fiber (red) and a spine head of the dendritic branch (green) ( n = 1). e 2D CLEM image showing fluorescence signals (red) of the GFAP-specific scFv probe overlapping with the cell body of a velate astrocyte in the granule cell layer ( n = 1). f EM image showing 2D segmentation (red) of the velate astrocyte in ( e ) ( n = 1). g 3D reconstruction of the velate astrocyte (red) labeled in ( e ) and two nearby granule cells (GC1 and GC2, light and dark blue) ( n = 2); the astrocyte extends a veil-like glial process (arrowhead) between the two granule cells. h EM image showing 2D segmentation of the glial process (arrowhead) between GC1 and GC2 ( n = 1). i 2D CLEM image showing fluorescence signals (red) of the GFAP-specific scFv probe overlapping with a Bergmann fiber ( n = 1). j EM image showing 2D segmentation (red) of the Bergmann fiber in ( i ) ( n = 1). k 3D reconstruction of two Bergmann glial cells (BG1 and BG2) ( n = 2) traced from their Bergmann fibers labeled by the GFAP-specific scFv probe. l EM image showing 2D segmentation of the cell body of BG2 ( n = 1) and a nearby basket cell ( n = 1), noting the lack of infoldings in BG2’s nuclear membrane compared to the basket cell. n indicates an example.

    Journal: Nature Communications

    Article Title: Multiplexed volumetric CLEM enabled by scFvs provides insights into the cytology of cerebellar cortex

    doi: 10.1038/s41467-024-50411-z

    Figure Lengend Snippet: a 2D CLEM image showing the fluorescence signal (green) of the calbindin-specific scFv probe overlapping with the cell body of a Purkinje cell. b EM image showing 2D segmentation (green) of the calbindin-positive Purkinje cell ( n = 1). c 3D reconstruction of the Purkinje cell labeled in a ( n = 1), with the cell body in dark green and a dendritic branch in light green; three parallel fibers (red) make synapses on three spine heads of the dendritic branch (arrow indicates a parallel fiber (PF); arrowhead indicates a synapse). d EM image showing 2D segmentation of the synapse (arrowhead) between a parallel fiber (red) and a spine head of the dendritic branch (green) ( n = 1). e 2D CLEM image showing fluorescence signals (red) of the GFAP-specific scFv probe overlapping with the cell body of a velate astrocyte in the granule cell layer ( n = 1). f EM image showing 2D segmentation (red) of the velate astrocyte in ( e ) ( n = 1). g 3D reconstruction of the velate astrocyte (red) labeled in ( e ) and two nearby granule cells (GC1 and GC2, light and dark blue) ( n = 2); the astrocyte extends a veil-like glial process (arrowhead) between the two granule cells. h EM image showing 2D segmentation of the glial process (arrowhead) between GC1 and GC2 ( n = 1). i 2D CLEM image showing fluorescence signals (red) of the GFAP-specific scFv probe overlapping with a Bergmann fiber ( n = 1). j EM image showing 2D segmentation (red) of the Bergmann fiber in ( i ) ( n = 1). k 3D reconstruction of two Bergmann glial cells (BG1 and BG2) ( n = 2) traced from their Bergmann fibers labeled by the GFAP-specific scFv probe. l EM image showing 2D segmentation of the cell body of BG2 ( n = 1) and a nearby basket cell ( n = 1), noting the lack of infoldings in BG2’s nuclear membrane compared to the basket cell. n indicates an example.

    Article Snippet: After adherence, cells were transfected with mammalian expression plasmids encoding mEmerald-tagged GFAP (Addgene #54107 or Flag-tagged human calbindin (Origene # RC201358) using Lipofectamine 2000 (Thermo Fisher # 11668500) or Lipofectamine 3000 (Thermo Fisher # L3000001) transfection reagent following the manufacturer’s protocol.

    Techniques: Fluorescence, Labeling, Membrane